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Yang Q. H., Wang Y. M., Tang X., Zhang Q. J., Dai S., Peng H. T., Lin Y. C., Tian Z. Q., Lu Z. Y., Chen L., Nano Lett., 2022, 22, 838 Representative western blot (A) and quantitative analysis (B) of regulatory proteins in Panc TuI (A) and BxPC3 (B) cells. pACC: Time‐dependent increase of ACC‐1 phosphorylation at Ser79 and of Raptor at Ser792 following incubation of Panc Tul (250μM GP‐2250) and BxPC3 (500μM GP‐2250). pRaptor: Time‐dependent Raptor phosphorylation at Ser 792 in PancTul and BxPc3 cells incubated with 500 and 1000μM GP‐2250. p53: Time‐dependent increase of protein level of p53 in Panc Tul and BxPC3 cells (500μM GP‐2250). Akt and mTor: Time‐dependent decrease of the protein level of Akt and mTor at 1000μM GP‐2250 in Panc TuI and BxPC3 cells. Bcl2: Time‐dependent downregulation of protein level of Bcl2 following incubation of Panc Tul and BxPC3 cells (500μM GP‐2250). ß‐Actin/HSP‐90 used as internal controls. Duke GJ, Santamaria J, Shann F, et al. Critical care outcome prediction equation (COPE) for adult intensive care. Crit Care Resusc. 2008;10:35–41. Moriyama Y, Watanabe E, Kobayashi K, Harano H, Inui E, Takeda K (2008) Secondary structural change of bovine serum albumin in thermal denaturation up to 130 °C and protective effect of sodium dodecyl sulfate on the change. J Phys Chem B 112:16585–16589Deiana M, Mettra B, Martinez-Fernandez L, Mazur LM, Pawlik K, Andraud C, Samoc M, Improta R, Monnereau C, Matczyszyn K (2017) Specific recognition of G-Quadruplexes over duplex-DNA by a macromolecular NIR two-photon fluorescent probe. J Phys Chem Lett 8:5915–5920 Data on adult patients receiving MV for at least 3 days in ICUs or non-ICU settings from April 2010 through March 2012 were obtained from the Quality Indicator/Improvement Project, a voluntary data-administration project covering more than 400 acute-care hospitals in Japan. We excluded patients with cancer-related diagnoses. Patient demographic data and the critical care provided were compared between groups. Results

Liu H. L., Chang L. N., Bai C. H., Chen L. Y., Luque R., Li Y. W., Angew. Chem. Int. Ed., 2016, 55, 5019

Kim S, Ohulchanskyy TY, Pudavar HE, Pandey RK, Prasad PN (2007) Organically modified silica nanoparticles co-encapsulating photosensitizing drug and aggregation-enhanced two-photon absorbing fluorescent dye aggregates for two-photon photodynamic therapy. J Am Chem Soc 129:2669–2675 Guo J., Qin Y. T., Zhu Y. F., Zhang X. F., Long C., Zhao M. T., Tang Z. Y., Chem. Soc. Rev., 2021, 50, 5366 An IBM 2285 Display Copier could be attached to the 2250 to provide 8½ by 11inch hard copy of the display contents under operator control. The mTOR complex 1 (mTORC1) drives cellular growth 23, 24 and can be directly inhibited by AMPK through phosphorylation of its constituent protein Raptor at Ser792. 11, 24, 25, 26

Fischer M, Georges J (1997) Use of thermal lens spectrometry for the investigation of dimerization equilibria of rhodamine 6G in water and aqueous micellar solutions. Spectrochim Acta A Mol Biomol Spectrosc 53:1419–1430What is 2250 Divided by 3 Using Long Division?. VisualFractions.com. Retrieved from http://visualfractions.com/calculator/long-division/what-is-2250-divided-by-3-using-long-division/. The IBM 2250 Graphics Display Unit was a vector graphics display system by IBM for the System/360; the Model IV attached to the IBM 1130. A reduction of the transcriptional activity of NF‐κB was apparent in both Panc Tul and BxPC3 cell lines by the change in expression of cyclin D1 and Bcl2. This is testimony to the functional relevance of NF‐kB inhibition by GP‐2250. The expression of cyclin D1, the driver of cell cycle progression, was reduced by GP‐2250 (Figure 5). This finding is in line with the previously shown reduction of the rate of cell proliferation by GP‐2250. 1 The expression of the anti‐apoptotic protein Bcl2 was likewise reduced by GP‐2250 (Figure 6). Thus, by inhibiting NF‐κB, GP‐2250 is able to disrupt tumour progression in a two‐pronged manner, by reducing the rate of cell proliferation and promoting apoptosis. Wang L, Zheng J, Yang S, Wu C, Liu C, Xiao Y, Li Y, Qing Z, Yang R (2015) Two-photon sensing and imaging of endogenous biological cyanide in plant tissues using graphene quantum dot/gold nanoparticle conjugate. ACS Appl Mater Interfaces 7:19509–19515

Yang WJ, Kim CH, Jeong M-Y, Lee SK, Piao MJ, Jeon S-J, Cho BR (2004) Synthesis and two-photon absorption properties of 9,10-Bis(arylethynyl)anthracene derivatives. Chem Mater 16:2783–2789Shen H. Y., Zhao H. H., Yang J., Zhao J., Yan L., Chou L. J., Song H. L., New J. Chem., 2022, 46, 3095 Zhong J. W., Yang X. F., Wu Z. L., Liang B. L., Huang Y. Q., Zhang T., Chem. Soc. Rev., 2020, 49, 1385 To test whether GP‐2250 might inhibit the NF‐kB pathway, p65/DNA binding was analysed in nuclear lysates prepared from Panc Tul and BxPC3 cells, which had been treated for 24h with various concentrations of GP‐2250. GP‐2250 caused a concentration‐dependent inhibition of p65/DNA binding in both cell lines, which was significant already at 250μM and reached 42.1% in Panc TuI (500μM) and 19.7% in BxPC3 (500μM). Bay 117082 served as control 18 (Figure 4C,D). In addition, when lysates from untreated Panc Tul and BxPC3 cells were incubated with the same concentrations of GP‐2250, a comparable degree of inhibition of p65/DNA binding was found (Figure 4E,F). This result supports the view that GP‐2250 is able to directly inhibit p65/DNA binding.