In gene regulation accessibility to promotor regions for the transcriptional machinery is now known be of major importance 1. Posttranslational modifications (PTMs) of the unstructured N-terminals of histones are important regulators of the accessibility, where important modifications are methylations and acetylations of lysine residues 2. Enzymes are classified as readers, writers or erasers when they read, introduce or remove accessibility controlling PTMs, respectively.

Lowary, P. & Widom, J. New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning. J. Mol. Biol. 276, 19–42 (1998). Pilotto, S. et al. Interplay among nucleosomal DNA, histone tails, and corepressor CoREST underlies LSD1-mediated H3 demethylation. Proc. Natl. Acad. Sci. 112, 2752–2757 (2015). Luger, K., Rechsteiner, T. J. & Richmond, T. J. Expression and purification of recombinant histones and nucleosome reconstitution. Methods Mol. Biol. 119, 1–16 (1999).Pouton, C. W. & Haynes, J. M. Embryonic stem cells as a source of models for drug discovery. Nat. Rev. Drug Discov. 6, 605–16 (2007). Georgala, P. A., Carr, C. B. & Price, D. J. The role of Pax6 in forebrain development. Dev. Neurobiol. 71, 690–709 (2011). Hartfield, E. M. et al. Physiological characterisation of human iPS-derived dopaminergic neurons. PLoS One 9, e87388 (2014). I left an opening for light wires under the Christmas Tree, however, if you don't plan on adding lighting the opening can be filled as shown in the instructions. The Winter Village train ride looks great with lights added (see photo below) however adding lights is beyond the scope of this modification and instructions for adding lights are not included with this kit.

Svergun, D. I. Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J. Appl. Crystallogr. 25, 495–503 (1992). Huang, Y. L., Walker, A. S. & Miller, E. W. A Photostable Silicon Rhodamine Platform for Optical Voltage Sensing. J. Am. Chem. Soc. 137, 10767–10776 (2015). Another nice variation are the decorations. Each tree has a unique style. The most exciting of the three is definitely the medium size, which has ball elements in turquoise and shiny silver for the first time, alongside heart elements more commonly found in Friends sets. Then the smallest one (this is starting to sound like the Christmas version of the Three Bears) is very European, featuring candle elements all across, while pearl-gold star elements act as interpretations of woven straw decorations.

Details

Choose either the single large Christmas Tree to make a grand statement - or the two smaller trees and light them both independently of each other with the two separate USB Power Cables included. 2-in-1 means twice the fun! KDM5B elution volumes were studied using a Superdex 200 5/150 Increase column (GE Health Care) using an HPLC system (Agilent 1100). KDM5B in concentrations in the range 0.05–2 mg/ml were applied using a buffer of 50 mM HEPES 300 mM NaCl pH 7.7 and 1 mM DTT and a flow rate of 0.25 mL/min. Samples were kept at 5 °C until the application to the column. The column was calibrated using standards from the LMW and HMW kits (Sigma A6103). SAXS Hopkins, J. B., Gillilan, R. E. & Skou, S. BioXTAS RAW: Improvements to a free open-source program for small-angle X-ray scattering data reduction and analysis. J. Appl. Crystallogr. 50, 1545–1553 (2017).

XML parts list for the Winter Village Train Ride For LEGO Set 40573 (Winter_Village_Train_Ride_Parts_For_LEGO_Set_40573_Parts.xml)Oh, S. et al. Combined Nurr 1 and Foxa 2 roles in the therapy of Parkinson’s disease. EMBO Mol. Med. 7, 1–17 (2015). Homology modelling was performed using the RaptorX server 37. Prediction of coiled coil regions from the amino acid sequence was done with the COILS server 38. EM data collection, 3D particle reconstruction and analysis Couture, J.-F., Collazo, E., Ortiz-Tello, P. A., Brunzelle, J. S. & Trievel, R. C. Specificity and mechanism of JMJD2A, a trimethyllysine-specific histone demethylase. Nat. Struct. Mol. Biol. 14, 689–695 (2007). Johansson, C. et al. Structural analysis of human KDM5B guides histone demethylase inhibitor development. Nat. Chem. Biol. 1–10, https://doi.org/10.1038/nchembio.2087 (2016). The Christmas Tree is easily attached to the center of the this build and the train itself can be moved freely around the ride.