Lee D, Kim EJ, Kilgore PE, Takahashi H, Ohnishi M, Tomono J, et al. A novel loop-mediated isothermal amplification assay for serogroup identification of Neisseria meningitidis in cerebrospinal fluid. Front Microbiol. 2015;6:1548. This study was limited due to the small number of malaria samples; hence, the performance of the sensitivity and specificity in clinical samples have not been demonstrated. The specificity in clinical samples that contained only P. vivax were evaluated. In addition, there were no C580Y mutations in k13 found among 34 P. falciparum isolates collected in 2010 in Indonesia. An accurate evaluation of the sensitivity and specificity of this method requires further investigation in a clinical setting.

Both the design and protocol of this study conformed to the Helsinki Declaration and were approved by the Institutional Ethics Committee. All samples were collected after written informed consent has been obtained. The methods of collection and analysis of the human samples were approved and cleared by the Institutional Ethical Review Board of Sam Ratulangi University and the University of Tokyo. DNA extraction from clinical samples Element in a biosensor, which converts the biorecognition event into an electrical measurable electrical signalKugelman JR, Wiley MR, Mate S, Ladner JT, Beitzel B, Fakoli L, et al. Monitoring of Ebola Virus Makona evolution through establishment of advanced genomic capability in Liberia. Emerg Infect Dis. 2015;21:1135–43. Y. Mori, K. Nagamine, N. Tomita and T. Notomi, Biochem. Biophys. Res. Commun., 2001, 289, 150–154 CrossRef CAS PubMed. And so far as the minion-themed bedding is concerned, there are a lot of options available in the market! If you like the one that is shown above, you can get this bedding set HERE. Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan O. Higgins, E. Clancy, M. Cormican, T. W. Boo, R. Cunney and T. J. Smith, Int. J. Mol. Sci., 2018, 19, 524 CrossRef PubMed.

Yamagishi J, Runtuwene LR, Hayashida K, Mongan AE, Thi LAN, Thuy LN, et al. Serotyping dengue virus with isothermal amplification and a portable sequencer. Sci Rep. 2017;7:3510. Imwong M, Suwannasin K, Kunasol C, Sutawong K, Mayxay M, Rekol H, et al. The spread of artemisinin-resistant Plasmodium falciparum in the Greater Mekong subregion: a molecular epidemiology observational study. Lancet Infect Dis. 2017;17:491–7. This is also referred to as clinical specificity and describes the percentage of negative test results which are correctly identified as negative 53 Well, since we already have a minion wallpaper, a minion bedsheet, curtains, and lights, why leave the rug out?Cuadros J, Perez-Tanoira R, Prieto-Perez L, Martin-Martin I, Berzosa P, Gonzalez V, et al. Field evaluation of malaria microscopy, rapid malaria tests and loop-mediated isothermal amplification in a rural hospital in South Western Ethiopia. PLoS One. 2015;10:e0142842.

Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Thung Phaya, Ratchathewi, Bangkok, 10400, Thailand A. S. Patterson, D. M. Heithoff, B. S. Ferguson, H. T. Soh, M. J. Mahan and K. W. Plaxco, Appl. Environ. Microbiol., 2013, 79, 2302–2311 CrossRef CAS.

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Y. Kouguchi, T. Fujiwara, M. Teramoto and M. Kuramoto, Mol. Cell. Probes, 2010, 24, 190–195 CrossRef CAS PubMed. Fig. 1 Percentage of publications listed in Web of Science (status as of 2019), refined search for appearance in title.

Patel JC, Lucchi NW, Srivastava P, Lin JT, Sug-Aram R, Aruncharus S, et al. Field evaluation of a real-time fluorescence loop-mediated isothermal amplification assay, RealAmp, for the diagnosis of malaria in Thailand and India. J Infect Dis. 2014;210:1180–7. There are various ideas for introducing a color palette to the room, the renowned one being by painting the walls. We’ll look at the alternatives in the further points. How About A Minion Bedding? Commercially available quartz crystals enclosed by electrodes and custom-made QCM system for LAMP and readout 153Ethical clearances were obtained from Institutional Ethical Review Board of Sam Ratulangi University and The University of Tokyo (Approval Number 10-49). The purpose of the study was explained in detail and written, informed consent was obtained from all participants. Confidentiality was maintained by avoiding the use of names and other identifiers. Funding J. Yamagishi, L. R. Runtuwene, K. Hayashida, A. E. Mongan, L. A. N. Thi, L. N. Thuy, C. N. Nhat, K. Limkittikul, C. Sirivichayakul, N. Sathirapongsasuti, M. Frith, W. Makalowski, Y. Eshita, S. Sugano and Y. Suzuki, Sci. Rep., 2017, 7, 3510 CrossRef. The recent spread of artemisinin (ART)-resistant Plasmodium falciparum represents an emerging global threat to public health. In Southeast Asia, the C580Y mutation of kelch13 ( k13) is the dominant mutation of ART-resistant P. falciparum. Therefore, a simple method for the detection of C580Y mutation is urgently needed to enable widespread routine surveillance in the field. The aim of this study is to develop a new diagnostic procedure for the C580Y mutation using loop-mediated isothermal amplification (LAMP) combined with the MinION nanopore sequencer. Results N. Nakamura, K. Ito, M. Takahashi, K. Hashimoto, M. Kawamoto, M. Yamanaka, A. Taniguchi, N. Kamatani and N. Gemma, Anal. Chem., 2007, 79, 9484–9493 CrossRef CAS. N. F. N. Azam, S. Roy, S. A. Lim and M. Uddin Ahmed, Food Chem., 2018, 248, 29–36 CrossRef CAS PubMed.