In One Bad Apple, Applejack and the Cutie Mark Crusaders meet Babs Seed at the Ponyville train station as Babs disembarks the Express. In the third act of For Whom the Sweetie Belle Toils, Sweetie Belle and her friends take the Express to Canterlot.

In Princess Twilight Sparkle - Part 1, Princess Twilight Sparkle's friends take the train from Canterlot back to Ponyville to prepare for the Summer Sun Celebration. Steamer" and the silhouette of "All Aboard" appear at the beginning of Rainbow Falls, "All Aboard" has a speaking role in Three's A Crowd, "Steamer" appears running Trenderhoof's train in Simple Ways, and "Steamer" and the silhouette of "All Aboard" appear at the beginning of Trade Ya! This means that Etsy or anyone using our Services cannot take part in transactions that involve designated people, places, or items that originate from certain places, as determined by agencies like OFAC, in addition to trade restrictions imposed by related laws and regulations. In the end of the same episode, the Mane Six take the train and share some of the treasures they received on their way back to Ponyville.In Hearthbreakers, the Apple family and Pinkie Pie take the Express to the Pie family rock farm for Hearth's Warming. They are officially unnamed, being referred to simply by their titles: "All Aboard" is Conductor in some episode credits, Gameloft's mobile game, and some episode scripts, is Conductor Pony in short credits, some episode scripts, and short script(s), is Ticket Agent in some episode credits and some episode scripts, and is Porter Pony in some episode credits and some episode script(s); "Steamer" is Train Conductor in credits and scripts.

The black locomotive version of the Friendship Express from Over a Barrel appears in Granny Smith's flashback in Family Appreciation Day. In The Crystalling - Part 1, the Mane Six, Spike, and Starlight Glimmer all take the train to the Crystal Empire to attend the Crystalling of the newborn Flurry Heart. The Princess Twilight Sparkle pony figure looks adorable in the conductor hat and glasses accessories, and the train car opens to play out fun stories inside and outside. An engine car, train car, and caboose each have rolling wheels that keep this My Little Pony Friendship Express Train rolling with fun! In Uncommon Bond, Sunburst takes the noon train from the Crystal Empire to Ponyville to visit Starlight, and takes the train back when his visit is over.

Hooves • Eclair Crème • Eiffel • Emerald Green • Felix • Fetter Keys • Fiddly Twang • Florina Tart • Gala Appleby • Globe Trotter • Golden Delicious • Golden Harvest • Goldengrape • Green Jewel • Haakim • Half Baked Apple • Hughbert Jellius • Jeff Letrotski • Joan Pommelway • Jonagold • Lady Gaval • Lavender Bloom • Lavender Fritter • Leadwing • Lilac Links • Lily Valley • Long Shot • Luckette • Lucky Clover • Lucky Star • Lyrica Lilac • MandoPony • Meadow Song • Minty • Mjölna • Mr. All Aboard and Steamer are the placeholder names of two male Earth ponies who work as Friendship Express train conductors. In Games Ponies Play, the Mane Six take the Express to the Crystal Empire to welcome the Equestria Games inspector. In Games Ponies Play, "All Aboard" has a brief uncredited speaking role when the Mane Six are boarding the train to the Crystal Empire, and later, "Steamer" has a speaking role by Andrew Francis [3] when the train gets there. In Just for Sidekicks, "All Aboard" has a speaking role by Peter New; he refuses Spike permission to board the train without tickets or chaperones for the Mane Six's pets.